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In the adult rat anterograde transport of enzymes and fluorescent tracers into the cell body of the basal forebrain cholinergic neurons was demonstrated in the septum and medial septal area. Experimental studies have shown that nerve fibre degeneration from a peripheral nerve can result in retrograde degeneration of the cell body in the target organ. We have examined whether a decrease in cholinergic afferent activity can result in retrograde degeneration of cholinergic neurons. A unilateral lesion of the nigral cholinergic cells of the forebrain in adult rats resulted in a loss of cholinergic innervation of the septum. Retrograde labelling was attempted using a retrograde axonal transport technique which is based on the uptake of a fluorescent retrograde tracer from the peripheral target organ by cell bodies containing the enzyme choline acetyltransferase (ChAT). ChAT-positive neurons of the medial septal nucleus could be retrogradely labelled with Fast blue when injected into the ipsilateral striatum or into the nucleus basalis of Meynert. Although in our experiments most retrogradely labelled medial septal neurons were also ChAT-immunopositive, we did not obtain any colocalization of Fast blue and ChAT in any of the labelled neurons. This suggests that retrograde transport of ChAT may be incomplete, or that the activity of ChAT in these cell
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To elucidate if heart failure affects the 24-h rhythm of heart rate, ten patients with severe stable cardiac failure due to coronary artery disease were studied in