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The meaning of “wouldn’t’
For example, the sentence I’m stuck in at the moment is “He wouldn’t offer you a job, would he?”
I don’t understand the meaning of “wouldn’t”.
This use of the word “Wouldn’t” is actually the same as “Couldn’t”. In this case, it is really saying:
He won’t offer you a job, will he? (and “didn’t offer” is just a
slight mis-use of the word, used with the meaning of the past
continuous, as in: “He hasn’t offered you a job, has he?”)
This use of this word could also be replaced with this word:
Prevent (something) from happening or being done
“They couldn’t get the air conditioning fixed in time for the party”
The meaning is essentially the same.
Proton (H+)-ATPases with different sensitivities to inhibition by bafilomycin, iodoacetate and vanadate.
Proton-ATPase from human erythrocyte lysosomes was dissociated into protomers which obtained two different steady-state Km values for ADP. The low Km values of about 6 microM are characteristic for the fast-stimulated ATPase and the high ones, of about 200 microM for the steady-state activity (which was stimulated during a strong centrifugation at low ionic strength). The inhibition of proton-ATPase by bafilomycin A1, an inhibitor of V-ATPase and insensitive to pH changes, indicated a different sensitivity to this compound. In contrast, the low ionic strength centrifugation technique gave high Km values in the presence of iodoacetate and the highly hydrophobic methanol, an inhibitor of E1P-bound